25 - The Anti-inflammatory Effect of Alloferon on Imiquimod-induced Psoriasis Through the Regulation of interleukin-22 Receptor Expression and Its Related Factors

Poster Board Number: 25
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Co-authors:

Jae Seung Kang - Seoul National University of Medicine; Yejin Kim - Seoul National University of Medicine; Seulgi Shin - Seoul National University of Medicine

Abstract Body : Objectives: Psoriasis is a mainly characterized by hyperproliferation of keratinocytes that thicken the skin with scaly plaque. In this study, we examined the regulatory role of alloferon on the development and progression of psoriasis in the imiquimod (IMQ)-induced psoriasis mouse model through the regulation of IL-22Rα expression.

Methods: For in vitro studies, TNF-α is treated to stimulate the human keratinocyte cell line, HaCaT, to mimic the psoriatic condition. For in vivo studies, imiquimod is treated to induce psoriasis in a mouse model.

Results: We have found that alloferon decreased IL-22Rα expression in the psoriasis-like keratinocyte treated with tumor necrosis factor (TNF)-α. It was observed that alloferon decreased epidermal hyperplasia in the IMQ-induced animal model through the downregulation of IL-22Rα. Although there was no significant difference in redness, a representative symptom of psoriasis, when IMQ was treated and changes in wild-type and IL-22Rα knock-out (KO) mice were compared, a decrease in acanthosis due to the defect of IL-22Rα expression in IL-22Rα KO mice was confirmed. Through this, it was confirmed that IL-22Rα is closely involved in the deterioration of psoriasis symptoms. Interestingly, however, it was also observed that the expression, such as IL-1β, IL-19, IL-33, and β-defensin, was suppressed regardless of IL-22Rα expression when alloferon was treated on IL-22Rα KO mice.

Conclusion: In this study, it is meaningful that alloferon showed an anti-inflammatory effect on psoriasis dermatitis by regulating the expression of IL-22Rα and genes related to psoriasis inflammation and pathology. Therefore, these results suggested that alloferon has therapeutic potential for psoriasis.