Hydro-dissection and Preservation Method in Teaching Palmar Anatomy : An Innovative Instrumentation in Anatomy for Chemical Free Dissection

Poster Board Number: 124
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Co-authors:

akashdeep Meshram - professor, Anatomy, Parul Ayurveda College

Abstract Body : Objective: 1:Need for an instrument aiding chemical-free preservation method
Objective: 2 A water current-aided blunt dissection method provides a transverse planar view of the dorsal, palmar, lateral and medial aspects of the hand for a more detailed view of the structures.
Objective :3 Develop an atlas of palm based on the observations of this technique.
Methodology: Development of a hydro-dissection and preservation equipment as mentioned below:
The whole unit was fabricated in stainless steel (SS) 304-grade matte finish, and is on castor wheels, two of them are free and two are lockable, mounted on tempered double glass and SS frame. The inner portion where the material for dissection is kept, is fabricated in 10/12 mm glass and fixtures. A quarter HP pump has been fixed externally. A refrigeration unit is installed under the water tank. The compressor is Emerson Copeland make, all piping is made of copper, and the condenser is fan-cooled with the best quality fan motor. A microprocessor-based digital temperature indicator is incorporated. Temperature is set at 2°C ± 1°C but if needed, it can be constantly maintained at 2°C. The overall dimension of the unit is W x D x H = 915 mm x 585 mm x 915 mm (36 inches x 23 inches x 36 inches). A specially designed manually operated specimen holding tray, which supports turbulent flow at various levels within the tank, the level of which can be adjusted by using a hook mechanism installed on to the walls of the tank.
A blunt dissection method using brushes with various bristle configurations (metallic, plastic, plant-based) aided by the turbulence of cold water thus creating a transverse planar view of the hand. The superficial fascia (dorsum) is identified as three layers and its extensiveness and intercalations with other structures were studied. Superficial and deep palmar structures were satisfactorily identified and their relations were also explored with maximum visibility The superficial veins and nerves. Muscles – thenar, hypothenar and other palmar muscles. Connective tissues were also delineated. Arteries and the branches along with the nerve were observed and traced up to the maximum visibility range. An atlas demonstrating the dissecting manual, including digital photographs and hand-drawn diagrams, was also prepared. A photographic and a diagrammatic atlas explaining the structures identified are prepared, the atlas also serves the purpose of a user manual of the HDPM technique.
Conclusion: The hydro-dissection and Preservation method can be used as an alternative as well as a frontier technique in Anatomy education and specimen making in undergraduate, postgraduate and clinical anatomy aspects.